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The thermal death point of a strain of Stapylcococcus aureus was determined to be 63°C.How would the the thermal death point of Bacillus subtilis compare?


A) The thermal death point of Bacillus subtilis would be just slightly higher than that of Staphylococcus aureus.
B) The thermal death point of Bacillus subtilis would be significantly higher than that of Staphylococcus aureus.
C) The thermal death point of Bacillus subtilis would be the same as that of Staphylococcus aureus.
D) The thermal death point of Bacillus subtilis would be lower that of Staphylococcus aureus.

E) C) and D)
F) All of the above

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Which is correct regarding the rate of microbial death?


A) Cells die at increasingly greater rates.
B) Only older cells die in a culture.
C) Cells in a culture die at a constant rate.
D) Upon contact with the control agent, all cells die at one time.
E) Cells become metabolically inactive but are never killed.

F) All of the above
G) B) and E)

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The use of a physical or chemical process to destroy vegetative pathogens on inanimate objects is


A) disinfection.
B) sterilization.
C) antisepsis.
D) sanitization.
E) degermation.

F) C) and D)
G) A) and B)

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__________ or steam sterilization is the process by which steam is heated under pressure to sterilize objects.

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Which is mismatched?


A) sodium hypochlorite-chlorine
B) iodophor-iodine
C) benzalkoniumchloride-quaternary ammonium compound
D) chlorhexidine-alcohol
E) formalin-formaldehyde

F) A) and B)
G) A) and C)

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All of the following are correct about the autoclave, except


A) it is important not to overload the chamber.
B) it is effective for sterilizing powders, oils, and waxy substances.
C) the duration of the process depends on how full the chamber is.
D) it is the temperature that kills the microbes, not the pressure by itself.
E) sterilization is achieved when steam condenses against the objects in the chamber and raises their temperatures.

F) A) and B)
G) D) and E)

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Which of the following types of control agents would be most likely to achieve sterility?


A) virucide
B) bactericide
C) germicide
D) sporicide
E) fungicide

F) B) and D)
G) A) and B)

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Physical methods of microbial control will typically sterilize, whereas chemical methods will only disinfect.

A) True
B) False

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Which of the following chemicals is a disinfectant for soft contact lenses?


A) hydrogen peroxide
B) alcohol
C) hexachlorophene
D) glutaraldehyde
E) hypochorites

F) C) and E)
G) C) and D)

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Alcohols


A) denature proteins when in a 50-95% solution.
B) are used to disinfect items by soaking.
C) are skin degerming agents.
D) at 50% or higher concentrations dissolve cell membrane lipids.
E) All of these choices are correct.

F) B) and C)
G) A) and E)

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HEPA filters are used to remove microbes from


A) air.
B) liquids.
C) human tissues.
D) medical instruments.
E) All of these choices are correct.

F) C) and D)
G) C) and E)

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Vials of microorganisms that undergo the freeze-drying process called _____ will remain preserved and viable for years.

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Dry heat


A) is less efficient than moist heat.
B) cannot sterilize.
C) includes tyndallization.
D) is used in devices called autoclaves.
E) will sterilize at 121°C for 15 minutes.

F) A) and B)
G) All of the above

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Bacteriostatic agents kill bacterial cells.

A) True
B) False

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Iodophors include


A) chloramines.
B) povidone iodine.
C) tincture of iodine.
D) alcohols.
E) chlorhexidine.

F) None of the above
G) A) and E)

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Which control method would not be a suitable choice for killing Mycobacteria in a capped culture tube?


A) ultraviolet (germicidal) light
B) gamma rays
C) 121°C at 15 psi for 15 minutes
D) 160°C for 2 hours
E) All of these choices are correct.

F) B) and E)
G) C) and D)

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Some microbial control agents are able to _____ cell proteins by breaking bonds that maintain the native state, three-dimensional configuration of the proteins.

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denature; ...

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The easiest microbial forms to kill or inhibit are


A) naked viruses.
B) vegetative bacteria and fungi.
C) endospores.
D) protozoan cysts.
E) Mycobacterium and Staphylococcus.

F) D) and E)
G) B) and D)

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The minimum sterilizing conditions in a steam autoclave are


A) 121°C at 15 psi for 15 minutes.
B) 63°C for 30 minutes.
C) 160°C for 2 hours.
D) 71.6°C for 15 seconds.
E) 100°C for 30 minutes.

F) B) and D)
G) C) and D)

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Chlorine compounds remain stable and effective in the presence of excess organic matter.

A) True
B) False

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